BOVIGAM™ TB Kit

The BOVIGAM kit is a blood-based assay of cellmediated immunity. Animals infected with Mycobacterium bovis (M. bovis) can be identified by measurement of the cytokine interferon gamma (IFN-γ). The BOVIGAM test is widely used as a stand-alone test, or as an ancillary test to the tuberculin skin test, and is approved by the OIE as a solution for all bTB testing situations.* The granted claims include use of the kit as a primary, stand-alone test and for the purpose of reestablishing bTB-free status after an outbreak.

Numerous studies underscore its effective use with the skin test in tuberculosis eradication programs. The test is used in many countries for the detection of M. bovis–infected cattle, sheep, goats, and buffalo (Syncerus caffer).

• Faster results—farm operations are back in business sooner than with skin testing

• Helps to minimize negative economic impact on the farm

• Very high analytical sensitivity

• Combinations of the Applied Biosystems™ BOVIGAM™ kit and stimulation antigens can help optimize every local bTB program

• Objective and highly reproducible

• Established technology

• Only one vet visit required (for blood sampling) compared to two for skin testing

- Microplates coated with IFN- γ:10 x 96 well plates with lids

-Positive control:     2 x 1 ml

- Negative control: 2 x 1 ml

with 0.01% w/v thimerosal.

- Green Diluent: Plasma diluent buffer. 1 x 60 ml

with 0.01% w/v thimerosal. Ready to use.

-Wash Buffer -  20X: 3 x 125 ml

with 0.01% w/v thimerosal.

-Conjugate – 100X. 1 x 1.5 ml

with 0.01% w/v thimerosal.

Blue Diluent: Conjugate diluent buffer –  5X. 1 x 25 ml

with 0.05% w/v thimerosal.

Enzyme Substrate Buffer. 1 x 125 ml

Ready to use

Chromogen Solution – 100X. 1 x 1.5 ml

Containing TMB in DMSO.

Enzyme Stopping Solution. 1 x 75 ml

(0.5M H2SO4)

Ready to use.