Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems, with CD indexes

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kits for Illumina™ are designed for the construction of high-efficiency DNA fragment libraries for whole-genome sequencing on Illumina™ next-generation sequencing (NGS) platforms. The kits support library preparation from a wide range of DNA samples and inputs (100 ng–500 ng) starting from intact DNA. In the Collibri™ PCR-free ES DNA library prep workflow, the enzymatic DNA fragmentation step is combined with dA-tailing in a one-vial protocol. Subsequent adaptor ligation reaction is carried out in the same vial followed by a size-selection step. Reduced number of sample transfer steps used in the workflow minimizes handling errors, and saves time and valuable sample. The entire library prep takes less than 2 hours, and does not require expensive equipment to shear DNA mechanically. For convenience, the kits provide color-coded components for visual tracking of library preparation progress. Inert dyes in the reagents do not interfere with enzymatic reactions and do not compromise library prep or sequencing results. The Collibri™ PCR-free ES DNA Library Prep Kits contain all the necessary reagents that are required for the preparation of up to 96 uniquely indexed DNA libraries, including enzyme mixes, dual-barcoded plate-format adaptors, and cleanup beads.

The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly