Cry3Bb LFS kit for Corn bulk grain ( 0.2 %)

Cry3Bb lateral flow strips (LFS) is intended to be used for the qualitative detection of Cry3Bb protein present in transgenic corn seeds carrying Monsanto Yieldgard Root worm RW event 863. The test detects one Cry3Bb positive corn seed in 500 conventional seeds (0.2%) The total incubation time of the assay is 10 minutes.

Hotline 024.3783.5922

An antibody specific to the Cry3Bb protein molecule is immobilized on the test line area of nitrocellulose membrane. Second antibody specific for Cry3Bb molecule is conjugated with colloidal gold nano particles and incorporated in the sample area of lateral flow strip. Anti mouse IgG is immobilized on the control line area of the LFS.

When the LFS is placed in the sample extract, the Cry3Bb protein present in the sample extracts binds to the antibody labelled with gold nano particles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of Cry3Bb, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.

SKU	AID 060
No. of tests	100
Max no. of samples	100
Specificity	Qualitative detection of Cry3Bb protein present in transgenic corn seeds carrying Monsanto Yieldgard Root worm RW event 863
Relevant species	Bulk grain corn
Cross-reactivities	The Cry3Bb LFS kit does not cross react with CP4EPSPS (Roundup Ready), Cry1F, Cry1Ac, eCry3.1Ab (AgriSure Duracade), mCry3A, Cry1Ab, Cry2A, Vip3, Cry34Ab1, Cry35Ab1 or PAT (LibertyLink).
Assay time	10 min
Storage	2 - 8° C
Kit components	Cry3Bb LFS strips 50 strips per canister:Two canisters per kit; One pack insert