GMOScreen RT 35S/NOS/ABII IPC (LR)

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

Hotline 024.3783.5922

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

The screening combination 35S/NOS/FMV covers a broad spectrum of genetically modified organisms but several important GMOs are still missing. The AgroBorder II assay offers much better coverage of relevant GMOs than the FMV-promoter test.

The multiplex kit GMOScreen 35S/NOS/ABII combines the detection of 35S, NOS and AgroBorder II and provides an optimal screening combination for GMOs from different plant species.

Benefits

All variants of the CaMV 35S promoter are detected
The AgroBorder II screening covers more commercialized GMOs than the FMV promoter detection
Multiplex kit for 45 samples per 96 well plate, three results per sample
No false-negative results: inhibition control (internal positive control; IPC) included in every reaction
Can be used flexibly with common DNA extraction methods

SKU	5421227101
No. of tests	96
Max no. of samples	45
Specificity	Cauliflower Mosaic Virus (CaMV) 35S promoter, A. tumefaciens nos terminator, AgroBorder II-Element
Relevant species	All GM plant species
Detection limit (LOD)	LODrel ≤ 0.01%, LODabs ≤ 10 copies
Time to Result	~2.5 h (~90 min PCR duration)
PCR cycler platforms	Applied Biosystems® 7500/Fast
Kit components	2x (20x) Qualitative real-time MasterMix GS-P-09.121 (IPC) for 35S/NOS/ABII detection, each composed of 650 µL BasicMix QL RT IPC (NR) GS-P-26.012 EFGi TP 2x (NR) 1.5U 390 µL OligoMix QL RT IPC (LR or NR) GS-P-09.121 35S/NOS/ABII 2x (20) 50 µL positive control DNA: plasmid DNAs pGE-P-09.121 (35S+NOS+ABII) (10 copies/µL) 1x (10x) 150 µL DNA stabilization buffer (for NTCs)

Product	Product description	No. of tests	Article No
GMOScreen RT 35S/NOS/ABII IPC (LR)	96 RT-PCR reactions, up to 46 samples per plate. Multiplex screening of p35s, tNOS, AgroBorder II with internal positive control	96	5421227101