Collibri™ NGS Library Prep Kit

1. Collibri NGS Whole-Genome Library Prep Kits

Invitrogen Collibri whole-genome library prep kits improve library prep success for high-throughput Illumina™ next-generation sequencing (NGS) systems.

• PCR-free protocols
• Best coverage of challenging genomic regions with the  physical protocol 
• Improved sensitivity of variant detection from 1 ng of DNA compared to Illumina, Kapa Biosystems™, or NEB™ kits
• Visual feedback for higher library prep success rates
• Protocols available for DNA that is physically sheared (PS) or enzymatically (ES).
• Accommodates a wide range of sample types and inputs: Intact DNA, Degraded DNA, including FFPE or cfDNA. Suitable for genomes of all sizes and consistent gene coverage from 1 to 500–1,000 ng
• Variant detection is consistent among low abundance (1 ng) and high abundance (500–1,000 ng) samples to confidently analyze variable samples.

2. Collibri Stranded RNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. mRNA can be converted to sequencing-ready library within 4.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detectio
• >98% strand specificity
• Efficient detection of non-coding RNA

3. Collibri™ Library Quantification Kit

The Invitrogen Collibri Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer. Collibri Library Quantification Master Mix includes Platinum II Taq Hot Start DNA Polymerase and enables convenient and accurate quantification of NGS libraries. The master mix contains all components needed for the amplification reaction, including primers and passive reference dye, and does not require any preparation before use. Due to the universal concentration of the passive reference dye in the master mix, the kit can be used with any qPCR instrument without adjustments.

Features of the Collibri Library Quantification Kit include:
• Accurate quantification of libraries made from various sample types, including degraded samples
• Ease of use with all components ready to use—no reagent preparation required
• Visual cues—specially formulated dyes included to track pipetting steps
• Platinum hot-start technology—superior specificity, sensitivity, and room-temperature reaction setup

4. Collibri™ Library Amplification Master Mix with Primer Mix

This Collibri Library Amplification Master Mix is supplied with a 10X Primer Mix that targets the P5 and P7 regions of Illumina adapters. The master mix is supplemented with an inert blue dye, while the primer mix contains a yellow dye. Mixing both components in a PCR reaction turns the final PCR solution green. This provides a visual aid when pipetting and helps decrease the risk of pipetting errors during reaction setup.

Platinum SuperFi DNA Polymerase has both 5’ to 3’ polymerase and 3’ to 5’ exonuclease (proofreading) activities, but lacks 5’ to 3’ exonuclease activity. Exceptionally strong proofreading activity ensures amplification of NGS libraries with supreme sequence accuracy, producing NGS error rates similar to PCR-free methods.

The proprietary reaction buffer contains all necessary PCR components and additives to enable efficient and uniform library amplification regardless of GC content, helping improve coverage across GC- and AT-rich sequences and other complex regions. High amplification efficiency enables the use of fewer cycles to achieve the desired yield.

Platinum hot-start technology inhibits DNA polymerase activity at ambient temperatures, allowing room temperature reaction setup and storage of pre-assembled PCR reactions for up to 24 hours prior to the PCR.