Library Quantification Kit for Next Genration Sequencing
Collibri™ Library Quantification Kit
Collibri™ Quantification Master Mix contains all the reagents that are required to amplify from Illumina™ adapter sequences: DNA polymerase, reaction buffer, dNTPs, ROX passive reference dye, and primers that target the Illumina™ adapter sequences—only the template needs to be added. Together with six pre-diluted DNA standards (a 10-fold dilution series of a 387-bp template) and Collibri™ Library Dilution Buffer, the kit is ideally suited for accurate quantification of NGS libraries between 150–1300 bp in length. Collibri™ Library Quantification Kit is compatible with any qPCR instrument, regardless of its ROX requirement due to the universal concentration of ROX passive reference dye in the Master Mix.
The Master Mix contains the Platinum™ II Taq DNA Polymerase, which is an engineered Taq DNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Polymerase activity is restored after the initial denaturation step at 95°C, providing an automatic "hot start". This offers increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature. Like standard Taq DNA polymerase, Platinum™ II Taq DNA Polymerase has both 5' to 3' polymerase and 5' to 3' exonuclease activities, but lacks the 3' to 5' exonuclease activity.
Colored components of the kit provide visual control of the proper workflow progress – when the blue Collibri™ Quantification Master Mix is mixed with the yellow Collibri™ DNA Standards and the yellow Collibri™ Library Dilution Buffer, the reaction mix becomes green. This allows the tracking of pipetting steps during reaction setup
Colors of the kit components have no influence on product performance and library quantification results.
| Component | 100 reactions | 500 reactions | Storage |
| Collibri™ Quantification Master Mix | 1.6 mL | 8 mL | –25°C to –15°C Protect from light |
| Collibri™ Library Dilution Buffer | 20 mL | 5 × 20 mL | –25°C to –15°C |
| Collibri™ DNA Standard 1 (20 pM) | 100 µL | 100 µL | –25°C to –15°C |
| Collibri™ DNA Standard 2 (2 pM) | 100 µL | 100 µL | –25°C to –15°C |
| Collibri™ DNA Standard 3 (0.2 pM) | 100 µL | 100 µL | –25°C to –15°C |
| Collibri™ DNA Standard 4 (0.02 pM) | 100 µL | 100 µL | –25°C to –15°C |
| Collibri™ DNA Standard 5 (0.002 pM) | 100 µL | 100 µL | –25°C to –15°C |
| Collibri™ DNA Standard 6 (0.0002 pM) | 100 µL | 100 µL | –25°C to –15°C |
| Name | Kit Size | Catalog Number |
|
Collibri™ Library Quantification Kit
|
100 Reactions |
A38524100 |
| 500 Reactions | A38524500 |
Corporate tax code: 0106092106 issued by Hanoi City Department of Planning and Investment on January 22, 2013.