The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time for labs running a variety of applications, including High Resolution Melting (HRM) analysis. Fully optimized for Fast cycling, the 7500 Fast System delivers high-quality results in as little as 30 minutes.
- Absolute Quantification
- Relative Quantification
- SNP analysis
- Melting Curve Analysis
- Plus/ Minus Assay
Demonstrated Performance | |
Dynamic range | 9 logs |
Sensitivity | Detection of 1 copy of template in a 20 µL reaction for a single reporter TaqMan assay, with 99.7% confidence. |
Run time | <30 minutes |
Thermal Cycling Syste | Peltier-based, 96-well block |
Optical Syste | CCD camera with halogen lamp excitation; five-excitation and five-emission filter |
Calibrated Dyes at Installatio | FAM TM/SYBR Green, VIC/JOE TM, NED TM/TAMRA TM/Cy3, ROX TM/Texas Red, and Cy5 |
Passive Reference Dyes | ROX |
Reaction Volume | 5-30 uL |
Sample format | Fast 96-well plates (100 μL); 8-tube strips (100 μL); optimized for 10 µL reactions |
Peak Block Heating Rate | 5,5oC/s |
Temperature range | 4 – 100oC |
Temperature Accuracy | +/- 0.25°C of setpoint/display temperature, measured at 3 minutes after clock star |
Temperature Uniformit | ± 0,5oC |
Dimensions (w x d x h) | 34 cm (13.99 in) x 45 cm (17.72 in) x 49 cm (19.29 in) |
Weigh | 34 kg (75 lb) |
Code | Description |
4365464 | 7500 Fast Real-Time PCR System, laptop, QST |
4360788 | 7500 Fast Real-Time PCR Systems Spectral Calibration Kit I |
4362201 | 7500 Fast Real-Time PCR Systems Spectral Calibration Kit II |
4351979 | TaqMan™ RNase P Instrument Verification Plate, Fast 96-well (for 0.1 mL block) |