7500 fast Real-Time PCR System

7500 fast Real-Time PCR System

Model: 7500 fast

Manufacture: Applied Biosystems™

The Applied Biosystems® 7500 Fast and 7500 Real-Time PCR Systems are versatile platforms for the detection and quantification of nucleic acids in standard 96-well formats. Our integrated real -time PCR solutions combine innovative thermal cycling systems, powerful software, optimized reagents, your choice of off-the-shelf or custom assays, and superior support for a variety of applications
 

The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time for labs running a variety of applications, including High Resolution Melting (HRM) analysis. Fully optimized for Fast cycling, the 7500 Fast System delivers high-quality results in as little as 30 minutes.
 

- Absolute Quantification 

- Relative Quantification 

-  SNP analysis

- Melting Curve Analysis

- Plus/ Minus Assay 

 

Demonstrated Performance
Dynamic range 9 logs
Sensitivity Detection of 1 copy of template in a 20 µL reaction for a single reporter TaqMan assay, with 99.7% confidence.
Run time <30 minutes
Thermal Cycling Syste Peltier-based, 96-well block
Optical Syste CCD camera with halogen lamp excitation;
five-excitation and five-emission filte
r
Calibrated Dyes at Installatio FAM TM/SYBR Green, VIC/JOE TM, NED TM/TAMRA TM/Cy3, ROX TM/Texas Red, and Cy5
Passive Reference Dyes ROX
Reaction Volume 5-30 uL
Sample format Fast 96-well plates (100 μL);  8-tube strips (100 μL)optimized for 10 µL reactions
 
Peak Block Heating Rate 5,5oC/s
Temperature range 4 – 100oC
Temperature Accuracy +/- 0.25°C of setpoint/display temperature, measured at 3 minutes after clock star
Temperature Uniformit ± 0,5oC
Dimensions (w x d x h) 34 cm (13.99 in) x 45 cm (17.72 in) x 49 cm (19.29 in)
Weigh 34 kg (75 lb)

 

Code Description
4365464 7500 Fast Real-Time PCR System, laptop, QST
4360788 7500 Fast Real-Time PCR Systems Spectral Calibration Kit I
4362201 7500 Fast Real-Time PCR Systems Spectral Calibration Kit II
4351979 TaqMan™ RNase P Instrument Verification Plate, Fast 96-well (for 0.1 mL block)

 

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