B ZERO® DON v2, ELISA

ELISA test kit for the quantitative detection of deoxynivalenol in cereals, feed and DDGS (Distillers).

Advantages: B ZERO^® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.

SKU	HU0040028, HU0040008
Assay principle	Microplate competitive enzyme immunoassay without standard curve. Sample quantification through interpolation of inhibition value on virtual curve (master-curve) provided for each batch of kit.
Format	Microtiter plate with 48 wells, 96 wells
Assay time	20 min (sample preparation not included)
Analysable samples	Cereals, feed, DDGS, wheat bran and middlings
Detection limit (LOD)	Wheat, Maize, Feed and DDGS - 0.04 ppm Durum Wheat - 0.12 ppm Wheat Bran and Middlings - 0.24 ppm
Sample preparation	Cereals (Maize, Wheat), Feed, DDGS - grinding, methanol-water extraction, filtration Durum Wheat - grinding, water extraction, centrifugation, dilution Wheat Bran and Middlings - water extraction, activated charcoal clean-up, filtration, dilution
Specificity	Deoxynivalenol 100% 3-acetyl-deoxynivalenol >100% 3-glucosyl-deoxynivalenol 64% 15-acetyl-deoxynivalenol 2% Nivalenol <4%