Superior quality and consistency for results you can rely on

Time-tested and trusted, our Gibco cell culture media line includes products designed to support the growth and maintenance of a variety of mammalian cells and cell lines. We’ve developed ready-to-use cell culture media products as well as powdered and concentrated liquid formulations to fit your experimental setup and your budget. Use the quick links below to access Gibco media.

DMEM

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

RPMI 1640

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

MEM

MEM (Minimum Essential Medium) can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Try Opti-MEM I Reduced-Serum Medium for greater transfection efficiency. This improved formulation allows for at least 50% reduction of FBS supplementation with no observable change in growth rate or morphology.

DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.