Novex pre-cast gel comes in two types:
Novex Tris-Glycine Gel is a polyacrylamide gel designed for electrophoresis of diverse samples based on traditional Laemmli chemical methods. Novex Tris-Glycine Gels provides highly efficient protein electrophoresis, even in samples containing high concentrations of detergents and salts. In addition, the Novex Tris-Glycine Mini Gel is also designed in WedgeWell format, making it easy to load samples with sample amounts up to 60 μL.
Novex Tricine Gel is a modified form of Tris-Glycine Gel, in which Tris-Glycine is replaced by Tricine in the Running Buffer. This gel is optimized for the separation of proteins and peptides with small molecular weight
Novex Tris-Glycine Gel
- High Efficiency: High proteolytic capacity for band stable quality
- Wedge-shaped well: Allows loading sample up to 60 μL.
- Rapid electrophoresis conditions: Allows rapid separation of your proteins in a constant electric field in no more than 60 minutes
- Flexible: SDS-free composition allows protein electrophoresis in the non-denatured or denatured form
Novex Tricine Gel
- Allows separation of proteins with molecular weights as low as 2.5kDa
- Suitable for direct protein sequencing application after dialysis transfer onto PVDF membrane
- Tricine Buffer system helps to minimize the variation of prrotein due to low pH
- Minimize protein loss during dialysis
Novex Tris-Glycine Gels | Novex Tricine Gels | |
Available polyacrylamide concentrations | 6%, 8%, 10%, 12%, 14%, 16%, 4–12%, 4–20%, 8–16%, 10–20% | 10%, 16%, 10–20% |
Available gel sizes | Mini (8 x 8 cm), midi (8 x 13 cm); 1.0 mm thick | Mini (8 x 8 cm), 1.0 mm thick |
Shelf life | Up to 12 months | 1–2 months at 2–8°C |
Separation range (denaturing) | 8 kDa to 250 kDa | 2 kDa to 20 kDa |
Running buffer | SDS-PAGE: Novex Tris-Glycine SDS Running Buffer Native-PAGE: Novex Tris-Glycine Native Running Buffer |
Novex Tricine SDS Running Buffer |
Sample buffer | SDS-PAGE: Novex Tris-Glycine SDS Sample Buffer Native-PAGE: Novex Tris-Glycine Native Sample Buffer |
Novex Tricine SDS Sample Buffer |
Recommend ladder | iBright Prestained Protein Ladder (western blotting ), PageRuler Plus Prestained Protein Ladder (gel staining) | Spectra Multicolor Low Range Protein Ladder (western blotting), PageRuler Unstained Low Range Protein Ladder (gel staining) |
Running time | 60 min | 90 min |
Novex Tris-Glycine Gels: https://www.thermofisher.com/vn/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/novex-tris-glycine-gels.html
Novex Tricine Gels: https://www.thermofisher.com/vn/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/specialized-protein-gels/tricine-protein-gels.html