Platinum™ Quantitative PCR SuperMix-UDG

Platinum® Quantitative PCR SuperMix-UDG is a ready-to-use cocktail containing all components, except primers, for the amplification and detection of DNA in real-time qPCR. The SuperMix combines the automatic “hot-start” technology of Platinum® Taq DNA polymerase with integrated UDG carryover prevention technology to provide optimal performance with a variety of qPCR detection technologies, including LUX ™ Fluorogenic Primers and TaqMan® probes (1–4). Volumes are provided for 100 or 500 amplification reactions of 50 μl each.

The SuperMix is supplied at a 2X concentration and contains Platinum® Taq DNA polymerase, Tris-HCl, KCl, 6 mM MgCl2, 400 μM dGTP, 400 μM dATP, 400 μM dCTP, 800 μM dUTP, uracil DNA glycosylase (UDG), and stabilizers.

• Highly sensitive and specific
• Allows room temperature reaction assembly
• Easy to use: just add template and fluorogenic primers or probes for detection
• Includes a vial of 50mM MgCl₂ to accommodate a variety of primer/template PCR systems
• Compatible with most real-time instruments

                                                                                100-rxn kit           500-rxn kit

Platinum® Quantitative PCR SuperMix-UDG          2 x 1.25 ml           12.5 ml

50 mM  Magnesium Chloride                                  1 ml                       2 x 1 ml

ROX Reference Dye                                               100 µl                     500 µl

Components may be stored at either -20ºC or 4ºC. ROX Reference Dye must be stored in the dark.