Platinum™ SuperFi™ II PCR Master Mix

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification. Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with innovative SuperFi II Buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.

• Features of Platinum SuperFi II PCR Master Mix include:
  • Exceptional >300X Taq fidelity
  • Universal primer annealing at 60°C
  • Superior specificity, sensitivity, and yields
  • Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement)

• 2 x 1.25 mL Platinum SuperFi II PCR Master Mix
• 2 x 1.25 mL water, nuclease-free
  
  Sufficient for 100 50-µL reactions

• High-fidelity PCR
• Cloning and sub-cloning
• Site-directed mutagenesis
• Amplification of GC-rich templates
• Template generation for sequencing
• High-throughput PCR
• Amplification of samples with suboptimal purity
• Long PCR
• Fast PCR