Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The 'hot start' property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

The hot-start property of Platinum Taq DNA Polymerase allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications. Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.

• Platinum™ Taq DNA Polymerase is a recombinant Taq polymerase complexed with a proprietary antibody that blocks the polymerase activity at ambient temperatures.
• Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic “hot start” and offering increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.
• This enzyme has a non-template-dependent, terminal transferase activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products.
• Like standard Taq, it has both 5' to 3' polymerase and 5' to 3' exonuclease activity, but lacks 3' to 5' exonuclease activity.

•  Platinum Taq DNA Polymerase
• 10X PCR Buffer (no magnesium chloride)
•  50 mM MgCl₂
•  KB Extender

Store at -10 to -30°C in a non-frost-free freezer.