PureLink™ Viral RNA/DNA Mini Kit

This quick reference sheet is included for experienced users of the PureLink™ Viral RNA/DNA Mini Kit.

Catalog number:12280050

Model:PureLink™

Brand::Invitrogen™ - Thermo Fisher Scientific

Hotline 024.3783.5922

The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The PureLink® Viral Mini Kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases, and is suitable for use in downstream applications that allow viral detection and genotyping

The PureLink® Viral RNA/DNA Mini Kit provides the following advantages:

• Rapid and efficient purification of high-quality viral nucleic acid using spin column–based centrifugation with no sample cross-contamination
• Specifically designed to purify viral RNA and DNA from ≤500 µL cell-free samples within 45 min
• Ability to elute viral nucleic acids in low elution volumes of 10–50 µL to allow sensitive downstream analysis

The components and amounts included in the PureLink™ Viral RNA/DNA Mini Kit are listed in the following table

Component	12280050
Viral Lysis Buffer (L22)	32 mL
Wash Buffer (WII) (5X)	15 mL
Proteinase K (20 mg/mL) in storage buffer (proprietary)	2 × 1.6 mL
Carrier RNA (lyophilized)	310 μg
Sterile, RNase-free Water (E3)	15.5 mL
Viral Spin Column và Collection Tube	50
Wash Tube (2.0-mL)	2 × 50
Recovery Tubes (1.5-mL)	50

Starting Material:	≤500 μL cell-free sample
Binding Capacity:	~5 µg nucleic acid
Column Reservoir Capacity:	700 μL
Wash Tube Capacity:	2.0 mL
Recovery Tube Capacity:	1.5 mL
Centrifuge Compatibility:	>10,000 × g
Elution Volume:	10–50 μL
Compatible application	qPCR, RT-PCR, Northern Blotting