TRIzol™ Reagent and Phasemaker™ Tubes Complete System

This system includes both the TRIzol™ Plus RNA Purification Kit and Invitrogen™ Phasemaker™ Tubes.

The TRIzol Plus RNA Purification Kit combines the strong lysis capability of TRIzol Reagent with the convenient and time-saving silica-cartridge purification protocol of the PureLink™ RNA Mini Kit to provide ultrapure total RNA typically within an hour from a wide variety of sample types.

Phasemaker Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of the TRIzol Reagent mix from the organic phase underneath, greatly simplifying the removal of the upper phase containing the RNA. This TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System is for the extraction of RNA, not DNA or protein. It will accommodate 50–100 mg of tissue. For cultured cells, please refer to the manual for information on sample size.

• Complete, ready-to-use reagent for the isolation of highquality total RNA
• Simultaneous isolation of RNA, DNA, and protein from a variety of biological samples
• Maintains integrity of RNA during sample homogenization or lysis while disrupting cells and solubilizing cell components
• Addition of chloroform, followed by centrifugation, separates the solution into an aqueous phase and an organic phase.
• RNA from aqueous phase is recovered by precipitation with isopropanol
• DNA from organic phase can be recovered by sequential precipitation
• Protein can be recovered from organic phase by isopropanol precipitation