|
Uncoated and antibody pair kits |
Coated ELISA kits* |
Instant ELISA kits |
ProQuantum kits |
Overnight coating required |
Yes |
No, pre-coated plate |
No, pre-coated plate |
No coating required |
Analytical sensitivity** |
<5 pg/ml |
<5 pg/ml |
<5 pg/ml |
<1 pg/mL |
Dynamic range** |
<7.8-500 pg/ml |
<7.8-500 pg/ml |
<7.8-500 pg/ml |
<1.6-5,000 pg/mL |
Sample volume |
10-100 µL |
10-100 µL |
25-50 µL |
2-5 µL |
Hands-on time |
1 hr 45 mins |
1 hr 20 mins |
20 mins |
30 mins |
Incubation time** |
Overnight |
2.5-4 hr |
4 hr |
1 hr |
Instrumentation needed |
Microplate reader, absorbance |
Microplate reader, absorbance |
Microplate reader, absorbance |
qPCR instrument |