Attune™ NxT Acoustic Focusing Cytometer
The Attune NxT Acoustic Focusing Cytometer 2019 Edition is an easy-to-use system for analyzing particle size and structure of cells as well as protein expression by fluorescent signals. Its groundbreaking system design delivers accurate results at speeds up to 10 times faster than similar systems. This analysis system allows us to minimize sample preparation operations, reduce analysis time, and allow an analysis of small sample sizes, minimizing errors leading to increased accuracy in the distribution of cell populations. Attune NxT Acoustic Focusing Cytometer is an ideal choice for immunological morphology, cell signaling, cell cycle, rare phenomena detection, stem cell analysis, cancer cell analysis, and apoptosis. In addition, bacteriological analyzes can also be performed using this system.
|
-The Invitrogen™ Attune™ NxT Flow Cytometer system combines precision with performance in a true benchtop flow cytometer, with up to 4 lasers and 16 parameters of detection. |
| - The device is designed in a modular form, with a fast and simple optional upgrade, suitable for a wide range of applications as well as a customer's budget. |
|
- The device uses a combination of ultrasonic waves (over 2MHz) and hydrodynamics to guide the cells into the correct position, focusing along the central axis in the capillary, for accurate analysis, without causing cell damage. |
| - With acoustic focusing technology alignment, the device achieves optimum sensitivity even at sample rates of up to 1 mL/min. |
| - Attune NxT is designed to capture data up to 35,000 events/second, detecting particles from 0.5µm to 50µm in size |
| - Equipment is capable of providing the following utilities: |
|
|
|
|
|
|
|
|
|
| Name | Unit | Quantity |
| - Attune® Acoustic Focusing Cytometer with Blue laser | Each | 1 |
| - Accessories included with the main unit: | Each | |
| • Power cord kit, universal voltage C13 2.5 m RC | Each | 3 |
| • Cable, USB 3.0 A-B M/M, 2 m RC | Each | 1 |
| • Cable, network RJ45 M/M CAT6 STP, 7 ft BLUE RC | Each | 1 |
| • 23-inch monitor | Each | 1 |
| • Dell™ computer (including mouse and keyboard) | Each | 1 |
| • Attune™ Performance Tracking Beads, 3 mL | Each | 1 |
| • Attune™ Wash Solution, 250 mL | Each | 1 |
| • Attune™ Focusing Fluid, 6 × 1 L | Each | 2 |
| • Attune™ 1X Shutdown Solution, 250 mL | Each | 1 |
| • Attune™ NxT Software license | Each | 1 |
| • Attune™ NxT Quick Reference Card | Each | 1 |
Attune NxT Acoustic Focusing Cytometer is an ideal choice for immunological morphology, cell signaling, cell cycle, rare phenomena detection, stem cell analysis, cancer cell analysis, and apoptosis along with many other bacterial analyses.
Optics: |
| - Laser power (depends on selected machine configuration) with options: |
| • Violet laser: 405nm, 50mW BSO, 100mW DP |
| • Blue laser: 488nm, 50mW BSO, 100mW DP |
| • Green laser: 532nm, 100mW BSO, 140mW DP |
| • Yellow laser: 561nm, 50mW BSO, 100mW DP |
| • Red laser: 637nm, 100mW BSO, 140mW DP |
| - Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection |
| - Laser profile: 10 x 50 μm flat-top laser providing robust alignment |
| - Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters |
| - Laser separation: 150 μm |
| - Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance required |
| - Onboard thermoelectric cooler: No warm-up delay; fiber isn’t affected by “on/off” |
| - Simmer mode: Instant “on/off” reduces usage and/or aging by 10x; only keep it “on” when acquiring samples; reports hours of usage |
| - Flat top specified at the flow cell: Coefficient of variation (CV) <3% over width of flat top |
| - Upgradable: Convenient field changes |
Fluidics: |
| - Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm |
| - Sample analysis volume: 20 μL to 4 mL |
| - Custom sample flow rates: 12.5–1,000 μL/min |
| - Sample delivery: Positive-displacement syringe pump for volumetric analysis |
| - Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm |
| - Fluid-level sensing: Active |
| - Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank |
| - Fluid storage: All fluids stored within instrument |
| - Extended fluidics option: Configuration for 10 L fluid |
| - Nominal fluid consumption: 1.8 L/day |
| - Automated maintenance cycles: ≤15 min start-up and shutdown—deep clean, sanitize, and debubble modes |
Performance: |
| - Fluorescence sensitivity: ≤80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤30 MESF for PE, ≤70 MESF for APC |
| - Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN) |
| - Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics |
| - Maximum electronic speed: 65,000 events/sec with all parameters |
| - Carryover: Single-tube format: <1% |
| - Forward and side scatter sensitivity: Able to discriminate platelets from noise |
| - Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood |
| - Forward scatter: Photodiode detector with 488/10 nm bandpass filter |
| - Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter |
| - Fluorescence detectors: 14 individual detectors |
| - Electronic pulse: Measured area, height and width pulse for all detectors |
| - Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise |
|
- Minimum particle size: 0.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories |
Software: |
| - Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells |
| - Flow rate: Precise flow rate control via software; no hardware adjustments |
| - Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec |
| - Overlays: Comparative analysis between samples; 3D view |
| - Sample recovery: System able to return unused samples |
| - Concentration: Direct concentration measurement without use of counting beads |
| - Software layout: Fully customizable for each user account |
| - Bubble detection technology: Stops automated run to preserve sample integrity |
| - Maximum single-event file: 20 million with option to append |
| - Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates |
| - Threshold: Up to 4 individual thresholds with user option to apply Boolean logic |
| - Gating: Hierarchal gating with the ability to derive gates. |
| - SmartGate™ labeling: Option to annotate quad gate names based on fluorophore and target names |
| - Voltage: User adjustable |
| - Window extensions: User adjustable |
| - Area scaling factor (ASF): User adjustable |
| - Acquisition settings: Documented in FCS files and maintained upon import |
| - Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously |
| - Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes |
| - Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software) |
| - User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count |
Data management: |
| - Software requirements: Invitrogen™ Attune™ NxT Software |
| - Monitor: 23-inch flat panel (1,920 x 1,080 resolution); dual-monitor capability |
| - Computer: Minitower desktop |
| - Operating system: Microsoft™ Windows™ 7 64-bit |
| - FCS format: FCS 3.1, 3.0 |
| - Processor: Intel™ Core™ i7 processor |
| - RAM: 16 GB |
|
- Hard drives: 80GB or more, and 250 GB RAID - compatible with all types of hard drives |
| Catalog number | Name | Unit size | Quantity |
| A24864 | - Attune® Acoustic Focusing Cytometer với Blue laser | Each | 1 |
| - Accessories included with the main unit: | Each | ||
| • Power cord kit, universal voltage C13 2.5 m RC | Each | 3 | |
| • Cable, USB 3.0 A-B M/M, 2 m RC | Each | 1 | |
| • Cable, network RJ45 M/M CAT6 STP, 7 ft BLUE RC | Each | 1 | |
| • Màn hình 23-inch | Each | 1 | |
| • Dell™ computer (including mouse and keyboard) | Each | 1 | |
| • Attune™ Performance Tracking Beads, 3 mL | Each | 1 | |
| • Attune™ Wash Solution, 250 mL | Each | 1 | |
| • Attune™ Focusing Fluid, 6 × 1 L | Each | 2 | |
| • Attune™ 1X Shutdown Solution, 250 mL | Each | 1 | |
| • Attune™ NxT Software license | Each | 1 | |
| • Attune™ NxT Quick Reference Card | Each | 1 | |
Attune NxT Flow Cytometer with available configurations |
|||
| A24858 | - Blue, red, yellow, violet (16 detection channels) | Each | 1 |
| A24859 | - Blue, yellow, violet (13 detection channels) | Each | 1 |
| A24860 | - Blue, red, violet (13 detection channels) | Each | 1 |
| A28993 | - Blue, red, yellow (12 detection channels) | Each | 1 |
| A24861 | - Blue, yellow (9 detection channels) | Each | 1 |
| A24862 | - Blue, violet (10 detection channels) | Each | 1 |
| A24863 | - Blue, red (9 detection channels) | Each | 1 |
| A24864 | - Blue (6 detection channels) | Each | 1 |
Hệ thống máy flow cytometry - Attune CytPix Flow Cytometer
Invitrogen Attune CytPix Flow Cytometer là thiết bị phân tích tế bào kết hợp sóng âm với camera tốc độ cao của Thermo Fisher Scientific vinh hạnh được Edison Awards công nhận là một trong những sản phẩm sáng tạo nhất của năm 2023.
Authorized distributor
VITECH Development Co., Ltd.
Corporate tax code: 0106092106 issued by Hanoi City Department of Planning and Investment on January 22, 2013.
Corporate tax code: 0106092106 issued by Hanoi City Department of Planning and Investment on January 22, 2013.