BACGene E. coli O157:H7

The BACGene E. coli O157:H7 kit provides materials for the rapid detection of Escherichia coli O157:H7 in food and environmental samples by targeting the O157 antigen-specific gene and the flagellar (H) antigen in a probe-based real-time PCR approach.

The BACGene E. coli O157:H7 kit is validated for use with the Agilent AriaMx™, Bio-Rad CFX96 Touch™ and CFX96 Touch™ Deep Well PCR platforms.

The kit is intended to be used by analytical laboratories and may also be applied for other purposes in the food product research and analysis field, e.g. microbial monitoring of production processes.

The kit is not intended for clinical diagnostics and should therefore be regarded as “For Research Use Only”.

Kit components:

For Lysis:

• 1x Lysis plate, empty, rippable (high profile).

• 1x Domed caps*, for use with lysis plate, set of 12 strips.

• 2x Lysis buffer S, 3 mL, store at -20 °C ± 2 °C or 4 °C ± 2 °C.

• 2x Proteinase K, to be mixed with Lysis buffer S, 500 μL, store at -20 °C ± 2 °C.

For PCR:

• 1x BACGene E. coli O157:H7 PCR plate, with pre-dispensed master mix and PCR support grid. Store at -20 °C ± 2 °C and protect from light.

• 1x Optical caps, for use with PCR plate, 12 strips.

• 2x E. coli O157:H7 positive control plasmid DNA, vial with yellow cap, 50 μL, store at -20 °C ± 2 °C. Do not freeze/thaw more than 6 times.