BACGene Mplex STEC Screen

E. coli O157:H7 is the most prominent serotype among STEC, however other serotypes are also potentially threatening to human health by expressing stx1 and stx2 (the Top6: O145, O121, O111, O103, O45 and O26).

The BACGene Mplex STEC Screen kit provides materials for the rapid detection of potentially shiga-toxigenic Escherichia coli (STEC) in food and environmental samples by analyzing stx1, stx2 and eae in a fast and reliable probe-based real-time PCR approach.

The BACGene Mplex STEC Screen kit is validated for use with the Agilent AriaMx™, Bio-Rad CFX96 Touch™ and CFX96 Touch™ Deep Well PCR platforms.

The kit is intended to be used by analytical laboratories and may also be applied for other purposes in the food product research and analysis field, e.g. microbial monitoring of production processes. The kit is not intended for clinical diagnostic and should therefore be regarded as “For Research Use Only”.

Kit components:

For Lysis:

• 1x Lysis plate, empty, rippable (high profile).

• 1x Domed caps, for use with lysis plate, set of 12 strips.

• 2x Lysis buffer S, 3 mL, store at -20 °C ± 2 °C or 4 °C ± 2 °C.

• 2x Proteinase K, to be mixed with Lysis buffer S, 500 μL, store at -20 °C ± 2 °C.

For PCR:

• BACGene Mplex STEC Screen PCR plate, with pre-dispensed master mix and PCR support grid. Store at -20 °C ± 2 °C and protect from light.

• 1x Optical caps, for use with PCR plate, 12 strips.

• 2x STEC positive control plasmid DNA, vial with yellow cap, 50 μL, store at -20 °C ± 2 °C. Do not freeze/thaw more than 6 times.