Collibri™ ES DNA Library Prep Kit for Illumina Systems, with CD indexes

Catalog number:A38605024; A38607096

Model:Collibri™ ES DNA Library Prep Kit for Illumina Systems, with CD indexes

Brand::Invitrogen™

The Invitrogen Collibri ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.

The Collibri ES DNA Library Prep Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng) starting from intact DNA. Additionally, a PCR-free version is offered for inputs starting at 100 ng DNA. The entire library preparation protocol takes about three hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

• Collibri ES DNA Library Prep Kit
• Collibri DNA Library Cleanup Kit
• Collibri DNA CD Indexes

Components	Cap/reagent color	24 preps	96 preps
Collibri™ ES DNA Library Prep Kit (Store at –20°C)
5X Fragmentation and dA-tailing Enzyme Mix	White	250 µL	1 mL
10X Fragmentation and dA-tailing Buffer	Blue	125 µL	500 µL
7X Ligation Master Mix for ES	Red	250 µL	1 mL
2X Library Amplification Master Mix for ES	Bue	1.25 mL	2 × 1.25 mL
Primer Mix	Yellow	500 µL	2× 500 µL
Collibri™ DNA Library Cleanup Kit (Store at 2°C to 8°C. IMPORTANT! Do not freeze.)
DNA Cleanup Beads	Orange	10 mL	30 mL
Wash Buffer (Concentrated)	Blue	4.5 mL	18 mL
Elution Buffer	White	5 mL	20 mL
Collibri™ DNA CD Indexes (store at –20°C)
Dual Index Adaptors (7 µM)		10 µL/well (24 wells)	10 µL/well (96 wells)

Giải trình tự Whole genome sequencing (WGS)
Resequencing
De novo assembly

Assay time	~160 min
Hands-on time	~45 min
Sample type	Low complexity dsDNA (bacteria/phage DNA) High complexity dsDNA (Mammalian, Mouse, Human, Rat, Plant)
Sample input amount	100ng– 500 ng DNA
Fragment size range	150 bp– 850 bp
Multiplexing	24 Combinatorial Dual (CD) indexes 96 Combinatorial Dual (CD) indexes 4 sets of 24 Unique Dual (UD) indexes (Set A, Set B, Set C, Set D)
For use with (equipment)	HiSeq™ 1000, HiSeq™ 1500, HiSeq™ 2000, HiSeq™ 2500, HiSeq™ 3000, HiSeq™ 4000, HiSeq™ X, MiSeq™, MiniSeq™, NextSeq™ 500, NextSeq™ 550, NovaSeq™ 6000
Sequencing application	Whole genome sequencing

Product name	Kit Size	DNA Index type	Catalog Number
Collibri™ PCR-Free ES DNA Library Prep Kit	24 preps	CD (Combinatorial Dual)	A38605024
Collibri™ PCR-Free ES DNA Library Prep Kit	96 preps	CD (Combinatorial Dual)	A38607096