Library Preparaion Kit for Illumina System (Physical Shearing)

Collibri™ PS DNA Library Prep Kit for Illumina Systems, with CD indexes

Catalog number:A38612024; A38614196

Model:Collibri™ PS DNA Library Prep Kit for Illumina Systems, with CD indexes

Brand::Invitrogen™

Hotline 024.3783.5922

Invitrogen™ Collibri™ PS DNA Library Prep Kits for Illumina™ are designed for the construction of high-efficiency DNA fragment libraries for whole-genome sequencing on Illumina™ next-generation sequencing (NGS) platforms. The kits support library preparation from a wide range of DNA samples and inputs (1 ng– 1 µg) starting from appropriately sheared or fragmented double-stranded DNA

The entire Collibri™ PS DNA library prep workflow is integrated into one vial, two-step protocol that takes less than 3 hours. The protocol does not require intermediate sample cleanup between End conversion and Adaptor ligation steps, which minimizes handling errors and saves time and valuable sample.

For convenience, the kits provide color-coded components for visual tracking of library preparation progress. Inert dyes in the reagents do not interfere with enzymatic reactions and do not compromise library prep and sequencing results. The Collibri™ PS DNA Library Prep Kits contain all the necessary reagents that are required for the preparation of up to 96 uniquely indexed DNA libraries, including enzyme mixes, dual-barcoded plate-format adaptors, and cleanup beads

Component	Cap/reagent color[1]	24 preps	96 preps
Collibri™ PS DNA Library Prep Kit (Store at –20°C)
2X End Conversion Master Mix	Blue	600 µL	2x1.2 mL
7X Ligation Master Mix for PS	Red	250 µL	1 mL
2X Library Amplification Master Mix for PS	Bue	1.25 mL	2 × 1.25 mL
Primer Mix	Yellow	500 µL	2× 500 µL
Collibri™ DNA Library Cleanup Kit (Store at 2°C to 8°C. IMPORTANT! Do not freeze.)
DNA Cleanup Beads	Orange	10 mL	30 mL
Wash Buffer (Concentrated)	Blue	4.5 mL	18 mL
Elution Buffer	White	5 mL	20 mL
Collibri™ DNA CD Indexes (Store at –20°C)
Dual Index Adaptors (7 µM)		10 µL/well (24 wells)	10 µL/well (96 wells)

The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Assay time	~160 minutes on average with library PCR amplification
Hands-on time	~45 minutes on average with library PCR amplification
Sample type	• Low complexity dsDNA (bacteria/phage DNA) • High complexity dsDNA (Mammalian, Mouse, Human, Rat, Plant), including challenging DNA samples (FFPE)
Sample input amount	1ng–1 µg of sheared or fragmented DNA
Sample input quality	Double-stranded DNA with A260/A280 ratio of 1.7–2.0
Fragment size range	150 bp–1000 bp
Multiplexing	24 Combinatorial Dual (CD) indexes 96 Combinatorial Dual (CD) indexes
System compatibility	iSeq™, HiSeq™ 1000, HiSeq™ 1500, HiSeq™ 2000, HiSeq™ 2500, HiSeq™ 3000, HiSeq™ 4000, HiSeq™ X, MiSeq™, MiniSeq™, NextSeq™ 500, NextSeq™ 550, NovaSeq™ 6000
Sequencing application	Whole-genome sequencing (WGS)

Name	Kit Size	DNA Index type	Catalog Number
Collibri™ PS DNA Library Prep Kit	24 preps	CD (Combinatorial Dual)	A38612024
	96 preps	CD (Combinatorial Dual)	A38614196